BACTERIOLOGICAL TECHNIC 39 



Solution for testing the formation of nitrates — 



MgS04 0.3 gram 



FeS04 0.4 gram 



NaCl 0.5 gram 



K2HPO4 0.5 gram 



Fused NajCOj i gram 



NaNOj I gram 



Distilled water 1,000 c.c. 



Dissolve 20 grams agar in the above solution. 

 Sterilize in the autoclave. The salt solution may also 

 be added to the silica jelly. For plating fill in culture 

 tubes. 



Solution for testing the assimilation of atmospheric 

 nitrogen — 



I. Solution 



KH3P04 I gram 



MgS04 0.50 gram 



NaCl o.oi gram 



FeS04 0.01 gram 



MnS04 o . 01 gram 



Dextrose. 20 grams 



Distilled water i,ooo c.c. 



Dissolve 20 grams agar in this solution if intended 

 for plating and tubing. Sterilize in the autoclave. 



2. Mannit Solution 



K2HPO4 o. 20 gram 



CaCIj 0.02 gram 



MgS04 o. 20 gram 



FejCk 0.01 gram 



Mannit 15 grams 



NaOH solution until neutral to -phenolphthalein 

 Distilled water 1,000 c.c. 



Dissolve 20 grams agar in this solution if intended 

 for plating and tubing. Sterilize in the autoclave. 



