42 LABORATORY GUIDE IN BACTERIOLOGY 



exercise 19. preparation of non-protein media 

 (synthetic media) 



Jordan's non-protein medium — 



Redistilled water 1,000 c.c. 



Asparagin 2 grams 



MgS04 I gram 



K2HPO4 ; I gram 



Dissolve and sterilize in the autoclave. 



Uschinsky's medium (Frankel's modification) — 



Water 1,000 c.c. 



Asparagin 4 grams 



Ammonium lactate 6 grams 



Na2HP04 2 grams 



NaCl S grams 



Dissolve and sterilize in the autoclave. 



Raulin's solution for the cultivation of molds — 



Distilled water 1,500 c.c. 



Cane sugar 70 grams 



Tartaric acid 4 grams 



Ammonium nitrate 4 grams 



Potassium carbonate 0.6 gram 



Ammonium phosphate 0.6 gram 



Magnesium carbonate 0.4 gram 



Ammonium sulphate 0.25 gram 



Zinc sulphate o . 07 gram 



Iron sulphate 0.07 gram 



Potassium silicate o . 07 gram 



Note. — Culture media should be stored in a dark, cool place. 

 If they are to be kept for a considerable length of time, the tubes 

 should be sealed with paraffin or with rubber caps. They should 

 be protected from dampness, as mold fungi are apt to alight on 

 the cotton stoppers and send their filaments into the tubes. 

 Before inoculation each tube should be examined, and those 

 which are cloudy or contain colonies of bacteria or molds should 



