44 LABORATORY GUIDE IN BACTERIOLOGY 



water. After solution add enough distilled water to 

 bring the volume to 300 c.c. 



Other useful stains are the following: 



Delafield's hematoxylin — 



Hematoxylin crystals 4 grams 



Alcohol 25 c.c. 



Ammonia alum 50 grams 



Water 400 c.c. 



Glycerin 100 c.c. 



Methyl alcohol 100 c.c. 



Alum hematoxylin — 



1. Hematoxylin 2 grams 



Absolute alcohol 100 c.c. 



2. Ammonia alum 2 grams 



Water 100 c.c. 



Mix I and 2 and add — 



Glycerin 850 c.c. 



Glacial acetic acid 100 c.c. 



Allow to stand for one month before using. 



Bismarck brown — 



Bismarck brown 0.5 gram 



Water joo c.c. 



Safranin — 



Safranin 0.5 gram 



Water 100 



c.c. 



Carbolic thionin blue (NicoUe) — 



Thionin blue i gram 



Carbolic acid 2.5 grams 



Water 100 c.c. 



Alum carmin — 



•'^i™ 2.5 grams 



Carmin i .0 gram 



Water 100 c.c. 



