TABLE OF CONTENTS 



Part I. Bacteriological Technic ^^^^ 



Section i. Laboratory Rules . 3 



Section 2. General Directions 4 



Section 3. Preparation and Cleaning of Glassware . 8 



Section 4. Methods of Sterilization 10 



Section 5. Preparation of Culture Media ... 18 



Section 6. Preparation of Staining Solutions . . 43 



Section 7. The Microscope 45 



Section 8. Scheme for Routine Study of Bacteria . 50 



Section 9. Method of Describing Cultures ... S3 



Section 10. Directions for rOling out Culture Charts 58 



Part II. General Bacteriology 



Section i. Preparation of Culture Media ... 69 

 Section 2. Collecting and Cultivating Micro-organ- 

 isms from the Air 70 



Section 3. Study of Molds, Yeasts, and Torulae ; 78 

 Section 4. Bacteriological Examination of Water, 



Air, and Milk 84 



Section 5. Exercises on Infection and Sterilization . 91 

 Section 6. Influence of Disinfectants, Light, and 



Heat on the Growth of Micro-organisms 94 



Section 7. Study of Chromogenic Bacteria ... 97 



Section 8. Study of Micrococci 100 



Section 9. Study of Intestinal Bacteria .... loi 



Part III. Important Pathogenic Bacteria 



Section i. Preparation of Culture Media . . . 105 



Section 2. The Pyogenic Group 105 



Section 3. The Group of Colon-Typhoid Bacilli 112 



Section 4. The Proteus Group . ... 121 



Section 5. The Capsulated Group 123 



Section 6. The Diphtheria Group 124 



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