FROST'S CULTURE CHART (MODIFIED) 



Group 



Name of organism 



Source 



Habitat 



References 



Morphological Characters 



< 



.2 



Sketches 



, Form: 

 a. On agar. 



Rod: 



b. On other media . 



Coccus: 



Spirillum 



2. Size 



3. Cell groupings. 



4. Staining powers 



a. Anilin gentian violet 



b. Laffler's methylene blue 



c. Gram stain 



d. Special stains, such as: (i) Flagella. . . (2) Spore . 

 (s^ "Tubercle" (4) Capsule 



5. Motility: (a) Flagellar (6) Molecular 



c. Character of movement 



6. Spores 



7. Special characters, such as: 



capsules, vacuoles, granules, 



pleomorphic and involution forms, etc 



. (b) Minimum . . . (c) Maximum . 



Physiological Characters 



1. Relation to temperature: (a) Optimum 



2. Relation to free oxygen: 



3. Relation to other agents such as: 



desiccation, light, disinfectants, etc: 



4. Pigment production: 



5. Growth in carbohydrate media: 



a. Stab or shake culture (dextrose agar) : gas production 



b. Fermentation tube: (z) growth in biilb:. . (2) growth in closed arm: 



c. Percentage of gas produced 



Dextrose 



Lactose 



Saccharose 



fermentation tube . 



After 24 hours 



After 48 hoars 



XT 



Gas formula ^qpr- in. . . 



d. Reaction (i) in bulb fa) in closed arm . 



6. Acid or alkali production (in litmus milk) 



7. Reduction of nitrates to nitrites , to ammonia 



8. Indol production; 24 hours 48 hours days 



fecal odor; 24 hours 48 hours days 



9. Enzym production; (a) proteolytic (b) coagulative. . . . (c) diastatic. . 



10. Characteristic odor 



11. Agglutination . .• 



12. Pathogenesis 



