SECTION I 



PREPARATION OF CULTURE MEDIA 



The following culture media are to be prepared for 

 this work: 



First Set 



Wort agar 20 tubes 



Ten of these tubes should contain about 7 c.c. for slants, the 

 other ten about 10 c.c. for plating. 



Wort gelatin 10 tubes 



Liquid wort 10 tubes 



Fermentatioa tubes 6 tubes 



Two of the fermentation tubes to contain i per cent dextrose, 

 two I per cent lactose, and the remaining two i per cent saccha- 

 rose. 



Second Set 



Meat extract agar 20 tubes 



Ten of these for slants and tea for plating. 



Pepton gelatin 15 tubes 



Broth 15 tubes 



Dextrose agar- iS tubes 



Litmus milk 15 tubes 



Synthetic potato 10 tubes 



The beerwort media are to be prepared during the 

 first week, the other media later. 



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