Il6 LABORATORY GUIDE IN BACTERIOLOGY 



Wells and Scott, Jour Infect. Dis., 1904, i, p. 72. 

 Gushing, Johns Hopkins Hospital Bull., 1900, p. 156. 

 Durham, Jour, of Exper. Med., 1900-1901, 5, p. 353. 



1. Routine study. — Observe the bluish-green colora- 

 tion of the cream ring in litmus milk, and make a 

 test for indol in Dunham's solution or sugar-free broth. 



2. Special study. — Inoculate plain sterile milk with 

 B. suipestifer. After 8-10 days it will be observed 

 that the milk is becoming transparent, due to a solvent 

 action of the alkali produced by the organism upon the 

 protein content. 



3. Special study. — Inoculate fermentation tubes as 

 with B. coll. Measure and analyze the gas. Compare 

 the results with those obtained in the study of the colon 

 group. 



4. Special study. — Inoculation of a rabbit sub- 

 cutaneously with B. suipestifer. Subcutaneous 

 inoculations of rabbits are made in the following 

 manner: An assistant, in a sitting position, places the 

 rabbit back down in his lap. The head projects be- 

 yond the knees of the assistant. The ears and hind- 

 legs are grasped, and the animal is thus held in position. 

 The hair is then cut off on a portion of the abdomen, 

 and the place is treated with mercuric chlorid and 

 alcohol. The skin is then pulled up, the syringe in- 

 serted, and the material injected. 



After the rabbit has died, study the lesions pro- 

 duced by the organism, and make smears from the site 

 of the inoculation, the heart's blood, and other organs. 

 Note the polar staining, i.e., stained portions at the 

 two ends of the cell and an unstained area between. 

 Make cultures on agar from the heart's blood and other 

 internal organs. 



