136 LABORATORY GUIDE IN BACTERIOLOGY 



3. Cultivation by Buchner's method, using fruit 

 jars. 



a) In a Mason fruit jar of ordinary type deposit 

 10 g. of pyrogallic acid. 



b) Smear vaselin around the mouth of the jar. 



c) Pour into the jar 100 c.c. of a i per cent solution 

 of NaOH. 



d) Deposit in the jar culture tubes previously in- 

 oculated. 



e) Fasten the cover of the jar, and incubate at 37° C. 

 for 48-72 hours. 



4. Cultivation in hydrogen gas. 



a) Inoculate all media from laboratory cultures. 



b) Fit up apparatus as shown in Fig. ^5. 



c) Place culture tubes in a Novy jar (Fig. 33, a). 



d) Open the faucet (6) of the gas generator (c), con- 

 taining zinc and hydrochloric acid. The hydrogen gas 

 generated is purified by passing through two jars, one 

 of which contains concentrated sulphuric acid id), the 

 other a 10 per cent solution of sodium hydrate (e). 

 Gradually the Novy jar is filled with hydrogen gas, 

 which can be ascertained by holding a culture tube 

 over the opening (/) and then over a burning match 

 or gas flame. As long as a detonation takes place the 

 hydrogen is mixed with atmospheric oxygen. When 

 the hydrogen in the Novy jar is pure, close it off by 

 turning the stoppers (g) and {b), and place the jar in 

 the incubator. The whole process occupies 10 or 15 

 minutes. 



5. A simple and effective method of anaerobic culti- 

 vation is as follows: Culture tubes, after inoculation, 

 are placed in a desiccation jar or any other jar with 



