MOLDS, YEASTS, TORULAE, AND BACTERIA 193 



6. Incubate one flask at room temperature, one at 

 37° C, and one at 40° C. 



7. Examine and note the degree of film formation 

 at the different temperatures and the differences in 

 morphology. 



8. Add small amounts of alcohol from time to time. 



9. Titrate dailywith 1/20 N • NaOH and determine 

 the amount of acid produced. 



EXERCISE 2 



Inoculate liquid wort to which 3 per cent saccharose 

 has been added with an alcohol-forming yeast. After 

 alcohol has been produced, inoculate with one of the 

 acetic-acid cultures and note the amount of acid 

 produced. 



