CONTENTS. 



CHAPTER I. 

 General Morphology and Biology. 



PAGE 



Introductory — Terminology — Structure of the bacterial cell — Reproduction 

 of bacteria- — ^ Spore formation — Motility- — Minuter structure of bacterial 

 protoplasm — Chemical composition of bacteria — Classification ■ — Food 

 supply — Relation of bacteria to moisture, gaseous environment, tempera- 

 ture, and light — Conditions affecting bacterial motility — Effects of bac- 

 • teria in nature — Methods of bacterial action — Variability among bacteria I 



CHAPTER II. 



Methods of Cultivation of Bacteria. 



Introductory — Methods of sterilisation — The preparation of culture mediae 

 The use of the culture media — The methods of the separation of aerobic 

 organisms — The principles of the culture of anaerobic organisms — Mis- 

 cellaneous methods — General laboratory rules 27 



CHAPTER III. 



Microscopic Methods — General Bacteriological Diagnosis 

 — Inoculation of Animals. 



The microscope — Examination of hanging-drop cultures — Film preparations 



— Examination of bacteria in tissues — The cutting of sections — Staining 

 principles — Mordants and decolorisers — Formula of stains — Gram's 

 method and its modifications — Stain for tubercle and leprosy bacilli — 

 Staining of spores and flagella — Observation of agglutination and sedi- 

 mentation — Routine bacteriological examination — Methods of inoculation 



— Autopsies on animals 87 



CHAPTER IV. 



Bacteria in Air, Soil, and Water. Antiseptics. 



Air: Methods of examination — Results. Soil: Methods of examination — 

 Varieties of bacteria in soil. Water ; Methods of examination — Bacteria 

 in water — Bacterial treatment of sewage. Antiseptics: Methods of 

 investigation — The action of antiseptics — Certain particular antiseptics . 123 



