DIAGNOSTIC INOCULATION. 235 



weeks. Unless the innoculated animals die before, they should 

 be killed at the end of six weeks and examined for lesions of 

 tuberculosis. 



According to Ostertag, the innoculations are best made 

 at the posterior inner surface of the thigh. This produces 

 results much sooner than the intraperitoneal method. Pseudo 

 tubercular processes are also avoided by this means. Experi- 

 mental animals may be killed for bacteriological examination 

 as soon as the lymph glands become conspicuous as painless 

 circumscribed tumors. This may occur in the course of ten 

 days, otherwise the animals should be killed for examination 

 by the end of the sixth week. 



II. Glanders. 



In view of the great infectiousness and incurability of 

 glanders, the object of the veterinarian is to determine the 

 presence or absence of this disease at the earliest possible 

 date. However, horses affected with glanders show no symp- 

 toms or at least no characteristic symptoms in the early stages 

 of the disease ; for this reason horses that have been 

 exposed to an infection with glanders 

 are subjected to a mallein test, with the 

 object of thus enabling us to recognize the disease. If the 

 animals show symptoms of the disease we endeavor to obtain 

 some of the pathological products or secretions and with them 

 inoculate experimental animals which are known from experi- 

 ence to be susceptible to the disease and develop it in a char- 

 acteristic form. 



Mallein inoculation. Mallein is the toxin of the bacilli 

 of glanders and is obtained from their cultures in a manner 

 analogous to that employed for obtaining tuberculin. The 

 crude preparation is a fluid, obtainable from the manufacturer 

 and injected in doses designated. It may also be obtained in 

 the dry or powdered form and is thus injected in doses of 0.02 

 — 0.1 G. according to the weight of the animal. It is best 

 to have the solution of the dry tuberculin prepared by the 

 manufacturer. 



