236 CLINICAL DIAGNOSTICS. 



Technique. This is the same as for tuberculin inocu- 

 lation. Taking temperature of animal to be tested, two or 

 three times at definite intervals before inoculation; inoculation 

 between 10-12 P. M., and taking temperatures again on next 

 day beginning at 5 A. M., and repeating every two hours until 

 6 P. M. 



Interpretation of Results. The International Veterin- 

 ary Congress has accepted the following principles for guid- 

 ance in interpreting the results of a mallein test: 



1. A positive reaction to mallein confirms the diagno- 

 sis of glanders only when it possesses a typical character. 



2. A typical reaction consists of an elevation of temper- 

 ature of at least two degrees centigrade [3.8° F.] and must 

 exceed 40° C. [104° F.] The temperature curve usually re- 

 mains at an elevation for some time or it may make a slight 

 drop and rise again on the same day. On the second, and 

 sometimes on the third day, there will be more or less eleva- 

 tion of temperature. A local as well as a general or con- 

 stitutional reaction is also observed. 



3. Elevations of temperature less than 40° C. [104° F.], 

 as well as greater elevations of an atypical character require 

 re-testing of the animal. 



4. Gradually attained high temperatures of considerable 

 duration, even if not typical, indicate the existence of glanders. 



5. A local typical infiltration of the tissues at the point 

 of inoculation is a positive indication of the existence of 

 glanders, even in cases where no thermal or general organic 

 reaction occurs. 



6. All malleinized animals, whether they react or not,- 

 must be subjected to the mallein test twice, at intervals of ten 

 to twenty days. 



The results of the mallein test cannot be compared with 

 those of the tuberculin test; they are less reliable. There is 

 no doubt that the varying results obtained from the use of 



