152 Artificial Parthenogenesis and FERTitizATioN 



6. The inhibitive effect of KCN and lack of oxygen upon the 

 activation of the unfertilized egg by bases suggested an investi- 

 gation of the influence of the various bases upon the rate of 

 oxidations in the unfertilized egg, to find out whether the weaker 

 bases raised the rate of oxidations more than the stronger ones.' 



The experiments were carried out on the unfertilized egg 

 of S. purpuratus. The oxygen content was determined ac- 

 cording to Winkler's method. The experiments were made 

 in a half grammolecular mixture of NaCl+KCI-FCaCl2 in that 

 proportion in which these three salts are contained in the sea- 

 water. 



We first give the results of a series of experiments in which 

 the relative influence of various bases was compared. The 



TABLE XXVI 



Number 



of 

 Experi- 

 ment 



Nature of the Solution 



mgm. of 



Oxygen 



Consumed 



Acceleration 

 of Rate of 

 Oxidations 



by tlie Base 



I. 

 II. 



{NsxitrSil 

 50 c.c. neutral+b.3 c.'c! N/io NaOH. '. '. 



so CO. neutrai+6.3 c.c. N/io tetra- 

 ethylammoniumhydroxide 



rNButral 



150 c.c. neutral-i-O.'s c.c. N/10 NH^Oh! 



/Neutral 



\60 c.c. neutral-l-0.3 c.c. N/10 tri- 

 methylamine 



/Neutral 



,.50 c.c. neutral+0.3 c.c. N/10 methyl- 



amine 



/Neutral 



150 c.c. neutral+0.3 c.c. N/10 ethyl- 



amine 



/Neutral 



\50 c.c. neutral+0.3 c.c. N/10 butyl- 



amine 



/Neutral 



\50 c.c. neutral+0.3 c.c. N/10 benzyl- 



amine 



0.28 

 0.40 

 0.15 



0.22 



1.43 

 1.50 



III. 



IV. 



0.30 

 0.81 

 0.40 



1.19 



2.70 

 3.00 



V. 



VI. 



VII. 



VIII, 



0.25 



1.18 

 0.28 



1.35 

 0.32 



1.23 

 0.22 



1.30 



4.70 

 4.80 

 3.80 

 5.90 



' Loeb and Wasteneys, Jour. Biol. Chem., XIV. 355, 1913. 



