30 



BACTERIOLOGY 



Iodine is used both solid and in solution. 



Chloroform is an important disinfectant, particularly for 

 sterilising blood-serum. 



Corrosive sublimate in solutions of 1 to 1,000 and 

 carbolic acid are necessary reagents for the laboratory 

 table. 



The oils employed are aniline, cedar, and those of 

 bergamot and cloves, used partly for clearing the microscopic 

 preparations, partly as solvents. 



For imbedding, a harder and a softer variety of paraffine, 

 and celloidine, are used. 



Canada balsam, and less frequently glycerine, are em- 

 ployed in the preparation of permanent microscopic objects. 

 The latter finds, however, its most extended application in 

 preparing nutrient materials. 



Gelatine, agar-agar, hlood-serum, albumen from the eggs 

 of hens and of insessorial birds, potatoes, starch, paste, 

 milk, rice, and bread are all used for making nutrient sub- 

 stances. Their application will be gone into in detail in 

 treating of culture-media. 



Stains. — The following is a list of the colouring matters 

 which are indispensable in making bacteriological pre- 

 parations : — Fuchsine, methyl blue, gentian violet, Bismarck 

 brown, methyl violet, malachite green, cosine, sa/ranine, and 

 dahlia are aniline colours which are sufficient for nearly all 

 investigations. Besides these, however, carmine, picro- 

 carinine, picric acid, hmmatoxyline, and Magdala red are re- 

 quired for preparations of tissues ; and for certain methods 

 of staining still other dyes are used, such as extract of 

 logivood,^ &c. 



Distilled water, alcohol, ether, xylol, and oil of turpentine 

 complete the equipment of reagents. 



Other utensils. — Platinum wires with loops sealed into 



' [Practically identical with Extractum HcBmatoxyli, B. P.] — Te. 



