294 BACTEEIOLOGi' 



Griibler's powder is dissolved in 80 c.cm. of water, and 15 

 c.cm. of a 0*5 per cent, solution of acid fuchsine is added. 

 The sections are stained for at least an hour, washed in 

 water for half a minute, placed in 95 per cent, alcohol for 

 one minute and then for two to five minutes in absolute 

 alcohol, and finally transferred to xylol and xylol balsam. 



. Sims Woodhead gives the following method of prepar- 

 ing and using this stain : — 5 c.cm. saturated solution of 

 methyl green, 10 c.cm. saturated solution of methyl orange, 

 and 2 c.cm. saturated solution of acid fuchsine are mixed, 

 after having first been diluted with about 40 volumes of 

 water each, to avoid the formation of a precipitate. Stain 

 for fifteen minutes to twelve hours, rinse in 1 per 1,000- 

 acetic acid, wash in dilute and then for one minute in abso- 

 lute alcohol, and finally immerse in xylol and benzol, and 

 mount in xylol balsam. 



The protoplasm of the epithelial cell is orange-red, the 

 nucleus green, the nucleoli brown or red ; the protoplasm 

 of the ' parasite ' is pale blue and the nucleus red. Con- 

 nective tissue is red, and leucocytes and all other cells are 

 orange-red with green nuclei. The protoplasm of the- 

 'parasite' is, however, sometimes orange-red, and the- 

 nucleus a little darker. Lastly, the living parasites may 

 sometimes be distinctly seen, according to Soudakewitch, 

 by examining scraped-off cancer cells in 0"6 per cent, salt 

 solution. 



(For bibliography, refer to the papers by Buffer and 

 Walker, and by Galloway.) 



Protozoa, in other forms of new growth. — Whether bodies 

 similar to those just described occur in sarcoma is still a 

 matter of doubt. Jackson Clarke, indeed, claims to have 

 found such, but the Morbid Growths Committee of the 

 London Pathological Society has decided ' that his supposed 

 ' See Rei^ort, Brit. Med. Journ., May 20, 1893, p. 1056. 



