32 BULLETIN 780, U. S. DEPARTMENT OF AGEICULTUKE. 



Tabm IX— Resistance qf Nosema apis to drying at room temperature.!, ( 



Date otinoculation. 



Results of iiioeul^tto^,5 



» 



July 26, 1916. 

 Sept. 11, 1914 

 Aug. 11, 1916 

 Oct. 2, 1914.. 

 Sept. 1, 1916. 

 Aug. 26, 1916. 

 Oof. 16, 1914. 

 May 24, 1916. 

 Sept. 1,1915.. 

 June 27, 1916. 



Nosema infection produced 



Do. 



Do. 



Do. 



Do. 



Do. 



Do. 

 No infectiqn produced: 



Do. 



Do. 



From results recorded in Table IX it will be observed that Nosem 

 apis drying at room temperature remained virulent for from 56 f< 

 60 days, that is, about 2 months. 



NOSEMA APIS DRYING AT OUTDOOR TEMPERATURE. 



Table X summarizes experiments in which Nosema apis was allowet 

 to dry at outdoor temperature. 



Table X. — Resistance Sf'N'osema apis to drying at outdoor temperature. 



Date of inoculation. 



Period of drying. 



Results of inoculatloii. 



Sept. 11, 1914., 

 Oct. 2, 1914.... 

 Aug. 17, 1914.'. 

 Ocf. 16, 1914... 

 June?, 1916... 

 Sept. 27, 1913.. 

 July 9, 1915.... 

 June 27, 1916.. 

 Aug. 17, 1915.. 

 July 17, 1916... 

 May 25, 1915... 

 July 17, 1914... 

 July 1,1914.... 



Months. 

 

 

 

 

 

 

 

 

 

 

 9 

 10 

 11 



21 

 42 

 46 

 56 

 60' 

 60 

 75 

 80 

 85 

 100 

 ■ 

 10 

 



Nosema infection produced, 



Do. 



Do. 



Do. 



Do. 

 No infection produced. 



Do. 



Do. 



Do. 



Do. > 



Do. 



Do. 



Do. 



The results recorded in Table X show that Nosema apis ceased to 

 be virulent after 2 months of drying at outdoor temperature. 



NOSEMA APIS DRYING AT REFRIGERATOR TEMPERATURE. 



Table XI summarizes experiments in which Nosema apis was 

 allowed to remain dry in the Refrigerator. ; ; .^ ; , 



Table "XT.— Resistance of Nosema apis to drying at refrigerator temperature.^ 



Date of inoculation. 



1i. : 



Dec. 2, 1915.. 

 Jan. 3, 1916.. 

 Mar. 3, 1916.. 

 Apr. 2, 1916.. 

 Apr. 22, 1916. 

 May 3, 1916... 

 July 3, 1916... 



Results of inoculation. 



Nosema infection produced 



Do. ' . 



Do. ', 



Do. i 



No infection produced. i 



Do. 



Do. 



e=itu?«M^ISa^rrt'oTppS.'M^^^^^^^^ 

 temperature, when present, however, at no time prevailed for more ttea a day. 



