34 



BULLETIIT 190, U. S. DEPAETMENT OF AGEICULTUBE. 



From experiments recorded in Table XII it was shown that Nosema 

 apis was destroyed by fermentation in 20 per cent honey solution at 

 incubator temperature in three days. 



FERMENTATION AT BOOM TEMPEHATUHB. 



In Table XIII are summarized experiments in which colonies were 

 inoculated with a suspension of Nosema apis in a 10 per cent sugar 

 (saccharose) solution, which had been allowed to ferment, at room 

 temperature. 



Table XIII. — Resistanee of Nosema apis to fermentation in sugar solution at room 



temperature. 



Bate of inoculation. 



Time of fermenta- 

 tion. 



Besults of Inoculation. 



•Sept. 8, 1915. 

 Sept. 9,1915. 

 June 4, 1915.. 

 Sept. 10, 1915 

 July 27, 1915. 

 July 29, 1915. 

 Sept. 13,1915 

 Sept. 15, 1915 

 Sept. 16, 1915 

 Sept. 1,1915. 

 Jan. 9, 1915.. 

 Sept. 15, 1914 

 Sept. 29, 1914 

 June 9, 1914.. 

 June 10, 1914. 

 May 13, 1915. 



MmChs. 

 

 

 

 

 

 

 

 

 

 

 

 



^ ? 

 10. 



18 



Days. 



S 



6 



11 



7 



8 



10 



10 



12 



13 



14 



18 



21 



34 



12 



Nosema infection produced. 



Do. 

 No infection produced. 



Do. 



Do. 



Do. 



Do. 



Do. 



Da 



Do. 



Do. 



Do. 



Do. 



Do. 



Do. 



Do. 



From Table XIII it wiU be seen that the parasite was destroyed 

 by fermentation in a 10 per cent sugar solution at room temperature 

 in from 7 to 1 1 days. The range of variation shown may be attributed 

 largely to variation in the temperature. 



FERMENTATION AT OUTDOOR TEMPERATURE. 



In Table XIV are summarized experiments made for the purpose 

 of obtaining approximate data relative to the resistance of Nosema 

 apis in a 20 per cent honey solution at outdoor tempeirature. 



Table XIY.— Resistance of Nosema apis to fennentation in a honey solution at outdoor 



temperature. 



Date of inoculation. 



July 26. 1916. 

 July 27, 1916. 

 July 28, 1916. 

 Sept. 8, 1915. 

 July 29. 1916. 

 Aug. 30, 1916 

 July 29, 1916. 

 Aug. 31, 1916 

 Sept. 2, 1916. 

 Sept. 6, 1916. 



Results of inoculation. 



I 



Nosema Infection produced. 



Do. 



Do. 



Do. 



Do. 



Do. 



Do. 



Do. 

 No infection produced. 



Do. 



