NOSEMA-DISBASE. 



35 



From Table XIV it wiU be observed that the parasite was destroyed 

 in 9 days in the presence of fermentation processes taking place in 

 a 20 per cent honey solution at outdoor temperature. 



At refrigerator temperature it was found that Nosemd apis resisted 

 fermentative processes for more than seven and less than nine days. 



It will be observed from the results obtained that Nosema apis 

 in the presence of fermentative processes is destroyed in a compara- 

 tively short time. The period, it will be seen, varies somewhat with 

 the temperature of the fermenting suspension. The experiments 

 tend to indicate, fm-thermore, that the time element depends slightly 

 upon the nature of the fermenting mediiun, the germ being destroyed 

 sooner in a honey solution than in a saccharose one. The time element 

 is dependent also upon the strength of the solutions employed. 



RESISTANCE OF NOSEMA APIS TO PUTREFACTION. 



Experiments have been made for the purpose of obtaining results 

 relative to the resistance possessed by Nosema apis to putrefactive 

 processes. The nature of the experiments was similar to those rela^ 

 tive to fermentation but instead of sugar solutions used for the 

 suspensions a 1 per cent peptone solution in water was employed. 

 In the experiments, suspensions, after undergoing putrefactive changes 

 at incubator, room, outdoor, and refrigerator temperatures, respec- 

 tively, were used in the inoculation of colonies. 



PUTREFACTION AT INCUBATOR TEMPERATURE. 



The experiments sumluarized in Table XV indicate the resistance 

 of Nosema apis to putrefaction at incubator temperature. 



Table XV. — Resistance of Nosema apis to putrefaction at incubator temperature. 



Date of Inoculation. 



Period of 

 putrefac- 

 tion. 



Results of inoculations. 



July 25, 1916. 

 July 26, 1916. 

 July 27, 1916. 

 July 28, 1916. 

 July 12, 1916., 

 Sept. 10, 1915. 

 July 15, 1916. 

 July 17, 1916. 



Days. 



2 

 3 

 4 

 S 



7 

 8 

 10 



Nosema infection produced. 



Do. 



Do. 



Da. 

 No infection produced. 



Do. 



Do. 



Do. 



By the results recorded in Table XV it is shown that Nosema 

 apis was destroyed by putrefaction at incubator temperatxire in 

 five days. 



