HOSEMA-DISEASE. 



39. 



In the experiments it was found that Nosema apis was destroyed 

 in all instances in which the temperature of the honey reached or 

 exceeded 140° F. (60° C), a temperature at which the germ is killed 

 by heat (p. 30). Sufficient data, thelrefore, have not been obtained 

 to warrant a definite conclusion regarding the time required for the 



Fia. 7.— Petri dish. Ttie top half is slightly raised, 

 used here are 4 inches in diameter. 



Those 



direct simHght to destroy Nosema q,pis suspended ia honey. The 

 results obtaiaed from tlie experiments made in which aqueous sus- 

 pensions were exposed to the sun give some idea as tp^*he probable 

 approximate time which would be required. 



PERIOD NOSEMA APIS REMAINS VIRULENT. 



PERIOD IN HONEY. 



In experiments made to determine the length of time Nosema apis 

 I remains virulent in honey a suspension of the parasite in honey was 

 I distributed in flasks, about one-haM piat being poured into each flask. 

 1 These were placed at room temperature and shielded from the light. 

 lAfter different intervals colonies were inoculated, the suspension' 

 ifrom a single flask being used. The results obtained are included 

 lin Table XX. 



Table, XX. — Period Nosema apis remains virulent in hmey. 



Date of inoculation. 



Besults of inoculation. 



Oct. 20, 1914. 



jFeb.4, 1915 



'Feb. 24, 1915 -.- 



((Feb. 4, 1915 



"Jan. 16, 1915 



4Julyl4, 1915 



'July 23, 1915 



J0ct.21, 1915 



'June 11, 1915 



Sept. 3, 1915..: : 



June 24, 1915 



JiOot. 21, 1915 



"July 24, 1916 



liAug. 14, 1913 



"May 1,1915 



Wune9, 1914 



'Apr. 27, 1915 



^ay 5, 1914.. .:..'... -J....:.'... ■:..:-. ;.:... i.-.- 



'ruly26, 1916 



Nosema infection produced. 



Do. 



Do. 



Do. 



Do. 

 No infection produced. 



Do. 



Do. 



Do. 



Do. 



Do. 



Do. 



Do. 



Do. 



Do. 



Do. 



Do. 



Do. 



Do. 



