26 THE BACTEEIA OF THE APIAEY. 



Bouillon.— This medium remains flour, with tbe formation of a friable white 

 sediment. Reaction neutral. 



Glucose.— The closed arm remains clear. No gas is formed. Reaction acid. 



Lactose. — Reaction neutral. 



Saccharose. — Reaction neutral. 



Levulose. — Reaction neutral. 



Maltose. — Reaction neutral. 



Mannite. — Reaction neutral. 



Agar. — A white, nonspreading growth occurs. 



Serum.— ^yhite, moderate, nonviscid, nonspreading growth occurs along the 

 surface inoculated. No liquefaction takes place. 



Potato water. — Reaction neutral. 



Potato. — Gray, luxuriant, fleshy growth occurs. 



Alilk. — No change occurs. 



Litmus milk. — No change occurs. 



Oelatim. — A moderate growth is formed, accompanied by no liquefaction. 



Acid agar. — Moderate growth takes place. 



Indol. — Negative. 



Nitrates. — Reduced to nitrites. 



Saccharomyces roseus. 



Occurrence. — Isolated from comb of healthy hive. 



Gelatin colonies. — Superficial colonies' are pink, convex, capitate, with lobate- 

 lobulate margin ; when magnified, the deep colonies are irregular, brownish- 

 yellow, and finely granular. 



Morpliology. — This cell is oval, attaining about 6.5,14 in length and 3.5/i in 

 thickness. The cells stain uniformly. 



Motility. — No motility occurs. 



Grain's stain. — The cells are not decolorized by Gram's stain. 



Oxygen requirements. — Aerobic. 



Bouillon. — This medium remains clear, forming a pink, friable sediment. A 

 pink band forms at the surface of the medium and adheres to the glass. 



Glucose.— ^The closed arm remains clear. No gas is formed. Reaction acid. 



Lactose. — Reaction neutral. 



Saccharose. — Reaction neutral. , 



Levulose. — Reaction slightly acid. 



Maltose. — Reaction slightly acid. 



Mannite. — Reaction neutral. 



Potato water. — Reaction acid. 



Glucose agar. — Luxuriant, red growth forms on the surface. 



Serum. — A pink, fleshy, nonspreading growth is formed, accompanied by no 

 liquefaction. 



Potato. — ^A thick, nonspreading, red growth occurs. 



Millc. — No apparent change takes place. The milk coagulates on boiling. 



■Litmus milk. — Reaction alkaline. 



Gelatin. — Moderate pink growth is formed, accompanied by no liquefaction. 



Aoid agar. — Slow growth occurs. 



Indol. — Negative. 



Nitrates. — Reduction to nitrites is positive. 



Saccharomyces G. 



Occurrence. — Found in the dead larvse of diseased adult bees. 

 Morphology.— Thej appear in hanging-drop preparation in large clusters 



