CONTENTS. 



Page. 



Introduction 7 



Technique 7 



Obtaining material for study 7 



Obtnining^ cultures 7 



Differentiation and identification of bacteria 9 



Tbe cultures which are described 9 



Morphology, staining properties, and oxygen requirements, with sug- 

 gestions on variations 9 



Media employed and suggestions as to the description of cultures 10 



PART I. BACTERIA OF THE NORMAL APIARY. 



Bacteria from the combs 13 



Bacteria from pollen 15 



Bacteria in honey and normal larvae 16 



Bacteria upon the adult bees 16 



Bacteria of the intestine of the healthy honey bee 18 



Saccharomyces and fungi 25 



Tabulation of micro-organisms normally present in the apiary 28 



Summary to Part I 29 



Bibliography to Part I 29 



PART II. THE DISEASES OF BEES. 



Brief history ^ 30 



The term "foul brood" as hitherto applied 31 



European foul brood (foul brood of Cheyne) 32 



Symptoms 32 



Confusion regarding foul brood in America 33 



The present investigation 34 



' Bacillus alvei 36 



Inoculation experiments 37 



Distribution of Bacillus alvei in infected hives 38 



Experiments with formaldehyde gas . 39 



American foul brood 40 



Symptoms 40 



The present investigation 41 



Bacillus larval 42 



The so-called " picljle brood" 43 



The so-called " blacic brood" 43 



Palsy or paralysis 44 



Summary to Part II 44 



Conclusions 45 



Bibliography to Part. II 46 



Index 47 



6 



