22 



BULLETIN 810, U. S. DEPAKTMENT OF AGRICULTUEE. 



rose) solution. A bit of soil was added to inoculate it further. 

 Records were made on suspensions fermenting at incubator and room 

 temperatures, respectively. Tables IX and X which follow sum- 

 marize experiments made : 



Table IX. — Bacillus pluton in a 10 per cent sugar solution fermenting at incu- 

 bator temperature 



Date of inoculation. 



Period of 

 fermen- 

 tation. 



Results of inoculation. 



Aug. 12, 1916 

 June 26, 1916. 

 Sept. 2, 1916- 

 Sept. 7, 1915. 

 Aug. 9, 1915. 

 June 30, 1916. 

 Julys, 1915.. 

 Aug. 24, 1915. 



Days. 

 3 

 5 

 7 

 8 

 10 

 15 

 15 

 24 



European foulbrood produced. 

 No disease produced. 



Do. 



Do. 



Do. 



Do. 



Do. 



Do. 



Table X. — Bacillus pluton in a .10 per cent sugar solution fermenting at room 



temperature 



Date of inoculation. 



Period of 

 fermen- 

 tation. 



Results of inociilation. 



June 30, 1916. 

 July 17, 1915. 

 Sept. 8, 1915.. 

 July 21, 1915. 

 Aug. 25, 1916. 

 Julys, 1916.. 

 Sept. 10, 1916 

 July 5, 1916. . 

 Aug. 26, 1916. 

 Aug. 3, 1915.. 

 Aug. 9, 1915.. 

 Aug. 25, 1915. 



Days. 

 9 

 ID 

 10 

 14 

 16 

 17 

 U 

 14 

 21 

 27 

 32 

 49 



European foulbrood produced. 



Do. 



Do. 



Do. 



Do. 



Do. 

 No disease produced. 



Do. 



Do. 



Do. 



Do. 



Do. 



The experimental results contained in Tables IX and X show that 

 Bacillus pluton is destroyed in a fermenting solution. At incubator 

 temperature the virus was destroyed in from 3 to 5 days, and at room 

 temperature it was killed in from 11 to 21 days. 



Similar experiments were made in which suspensions in 20 per 

 cent honey solutions were allowed to ferment at outdoor temperature. 

 The records obtained show that Bacillus pluton in this environment 

 was still alive and virulent after one month. 



RESISTANCE OF BACILLUS PLUTON TO PUTREFACTION 



Suspensions of the contents of stomachs from larvaj sick or dead 

 of European foulbrood were made in a 1 per cent peptone solution. 

 Soil was added to inoculate it further. Putrefactive changes were 

 allowed to take place at incubator and room temperatures, respec- 



