EUROPEAN FOXJLBROOD. 



23 



tively. In Tables XI and XII. which follow, are summarized the 

 experiments performed : 



Tablm XI. — Bacillus [ilutoii in the presence of putrefactive processes at incubator 



tciiiperatiirc 



T>i\io of inoculation. 



June 30, 1916. 

 Sept. 2, 1916.. 

 Aug. 15, 1916. 

 Sept. 7, 1915.. 

 Julys, 1916.. 

 Sept. 10, 1915, 

 Julys, 1916... 

 Aug. 23, 1915. 

 Aug. 30, 1915. 



Table XII. — Bac-illioi pluton in titc presence of putrefactive processes at room 



temperature 



Date of inoculation. 



Period of 

 putrefac- 

 tion. 



Results of inoculation. 



Aug. 4, 1914. 

 July 17, 1915. 

 July 5, 1916.. 

 July 23, 1915. 

 Aug. 14, 1914. 

 Sept. 17, 1915 

 Aug. 25, 1916. 

 Sept. 2, 1916. 

 Aug. 3, 1915.. 

 Aug. 28, 1916. 

 Sept. 1,1914. 

 Sept. 16, 1914 

 Aug. 12, 1916. 



Days. 



European foulbrood produced. 



Do. 



Do. 



Do. 



Do. 



Do. 



Do. 



Do. 



Do. 

 No disease produced. 



Do. 



Do. 



Do. 



As shown by Tables XI and XII Bacillus pluton is destroyed in 

 the presence of putrefactive processes. At incubator temperature 

 it resisted the* effects of these processes for from 7 to 13 days and 

 at room temperature for from 21 to 35 days. 



During August and September, 1916, preliminary experiments 

 were made testing the resistance of Bacillus flvion to putrefaction 

 at outdoor temperature. The parasite was alive and virulent after 

 40 days. The maximum period during which it will remain so has 

 not been determined. 



VIABILITY OF BACILLUS PLUTON IN HONEY 



Honey suspensions of Bacillus pluton from the stomach contents 

 of larvse sick or recently dead of European foulbrood were made and 

 distributed in flasks each containing about 300 c. c. These were 

 allowed to stand at room temperature shielded from the light. At 

 intervals thereafter colonies fre6 from the disease were inoculated 



