Uay 1, 1036 



Spores <yf Bacillus Larval in Honey 



699 



The foregoing process gives a concentration of spores in the sedi- 

 ment from the 5-cc samples of honey suspended in 0.1 cc of water, 

 or one-fiftieth the original volume. 



METHOD OF COUNTING SPORES 



A method simUar to that of Breed and Brew * for counting bacteri'a 

 in milk was used for counting the spores of Bacillus larvae in these 

 staiued smears. This method is similar to that described in a previous 

 paper ® and is represented by the formula 



Number of spores per cubic centimeter= 



KNXXIOOXD 



N 



where K is the factor for the number of circular fields per 1-cm^ area, 

 N is the number of circular fields counted, X is the actual mean 

 number of spores per field, 100 is the factor that gives the number of 

 spores per cubic centimeter from 0.01 cc of the suspension, and D 

 is the dilution. 



Table 1. — Spore counts in stained smears of the sediments resulting from the cen- 

 irifuging of duplicate 5-cc portions of five samples of honey containing known 

 numbers of spores of Bacillus larvae 



' A and B represent duplicate portions of the samples. 



' Bebed, B. S.; aitd Sbkw', J. li. fcocNTJNa baoieeia bt means of ihe micSoscope. N. Y. StSte 

 Agr,, Expt. eta. TSoH, Bull. 4fl, 31 pp.. Ulus. 1916. 

 ' STTETEtANT, A. F. SeB foOtndt^ 3. 



