The Sterilization of American Foulbrood Combs 11 



expensive for practical use, was also tested. Only four series, each 

 of seven cells, were tried with this carrier for purposes of oora- 

 parison, two of open and two of sealed cells, the proportions used 

 being 50 parts of iso-propyl alcohol, 30 parts or water, and 20 

 parts of formalin. Of all the 28 cells cultured only one sealed cell 

 was found to contain viable Bacillus larvae. This solution is there- 

 fore in about the same class as that of acetone. 



MISCELLANEOUS SOLUTIONS 



The results of experiments with these solutions are presented in 

 Table 3. The use of iodine solutions proved entirely impracticable 

 for sterilizing infected combs. In dilutions of 1 to 50,000 in water 

 and even 1 to 500, with an immersion of 48 hours, no germicidal 

 action on the spores from diseased material in either Open or sealed 

 cells could be demonstrated. A solution of 1 to 20 iodine killed all 

 the spores, both in open and sealed cells, but the comb was attacked 

 to such an extent as to make it too soft for further use. The probable 

 reason for the lack of germicidal action on the part of iodine is 

 that this substance combines readily with the fatty-acid constituents 



Table 3.-^Gultural results of various tests with samples of comb treated for 1 

 hours in various solutions 



Composition of solution 



g-9 



— ' 3 



§■32 

 la 



Open cells 



O-t-a 

 CO Vi 



1^3 



SboS 

 S.9l 

 ■a ten 



lie 



Sealed cells 



P 



o 



I. 



bOJ3 



II 



m 



T! 9" 

 s43 d 



lodtne, 1-50,000 water 



iodine, 1-600 water 



bdine, 1-20 water 



lieiiatnred alcohol (50 per cent), 93 

 , c. «., hydrocbloric scid, 6 c. c, 



formalin, 2 c. c. 

 Ijenatured alcohol, 90 c. c, hydro- 

 ; diloric acid, Sec. tormaun, 6 c. a. 

 -B^anatored alcohol (50 per cent), '93 



c. c, hydrochloric acid, 5 c. c, 



formalin, 2 c. c. 

 ;;^niatared alcohol, 90 c. c, hydro- 



ehlorlc add, 6 c. c, formalin, See. 

 'Jtoetle add, 10 c. c, water, 70 c. c, 



formalin, 20 c. c 



Do — 



Acetic acid, 6 c. c, water, 76 c. c, 



formalin, 20 c. c. 



Do... 



Denatured alcohol, 80 c. c, formalin, 



20 e. c, glycerin, 2 c. c. 



Do.... 



1 per cent solution of gelatine, 80 



■e. 0., formaUn, 20 c. c. 



Do 



10 per cent ketone solution added to 



9S per cent alcohol,80 c. c, forma- 

 Un, 20 c. C: 



Hmirs 

 48 

 4S 

 48 



First., 

 ...do.. 



Third... 



First... 



First. 

 Do. 



Do. 



Do. 

 Do. 



Do. 



Fourth. 



Do 

 Do. 



Second. 

 First. 



Third. 

 Second. 



Do. 

 Do. 



' One showed Savr spares germinated. 



' Few spores germinated. 



