18 Department Circular 28i, U. S. Dept. of Agriculture 



was apparently still incomplete, probably because a trapped air 

 bubble had prevented sufficient solution froni passing through the 

 opening. 



Table 7.- 



-Cultural results of various tests with samples of comb treated for 48 

 hours in commercial alcohol-formalin solution 



3 q3 CI 



ial 



"at" 



Open cells 



■a 



o 



o 



35 



ll 



o 



" 5 

 o 



3 "■3 



Sal 



Sealed cells 



> boo H 



OQ'O 



1.-. * 



■S3 



II 

 o 



|5 



I-. 

 ■3 

 o 



s-si 





.aal 



Preliminary tests.. 



Open (uncapped) cells compared with sealed cells; 



used solution. 

 Open (uncapped) cells compared with sealed cells; 



fresh solution. 

 Fresh solution; control for samples washed after 



treatment. 



Open cells, washed in water after treatment 



Cells with perforated cappings compared with 



open cells. 



4 

 3 

 3 



7 



7 



8 



6 



7 



7 



10 



10 



10 



ID 



10 



10 

 10 

 10 



First., 



First 

 Second. 

 SV8t.,- 

 ftfth. 

 ThiEd. .. 

 FireT 

 Thifd- 

 Secoqd. 

 First. 

 Ninth. 

 Third. 

 Do. 

 First. 



Tenth. 



I One showed few spores germinated. 

 • Few spores germinated. 



• Three showed few spores germinated. 

 ' Eight showed few spores germinated. 



Table 8. — Cultural results of various tests loith samples of comb treated for i 

 hours vn, commercial alcoJiol-formaUn solution 



Preliminary tests 



Uncapped cells only; used solution 



Uncapped cells only; fresh solution 



Fresh solution; control for samples washed after' 



treatment; imcapped cells only. 

 Open cells, washed in water after treatment.. 

 Cells with perforated cappings compared with 



open cells. 



<s S3 S 



Open cells 



11 



■a 



II 

 O 



Is 



111 



a o fl 



CO s . 



XI go 



8 ^3 



•u boo H 



First.. 



Tenth.. 

 Sixth... 



.First- 



Sealed cells 



■a 





35 



.3 

 1^ 



« o 



3 

 o 





First. 



Eighth. 

 Fourth. 



' Few spores germinated. » One showed few spores germinated. • Two showed few spores germinated. 



