The Sterilization of American Foulbrood Combs 



19 



Table 9. — Cultural results of various tests loith samples of comb treated for 48 

 hours in water-formalin solution 



PreHminary tests 



Open (uncapped) cells compared with sealed cells; 



used solution. 

 Open (uncapped) cells compared with sealed cells; 



fresh solution. 

 Fresh solution; control for samples washed after 



treatment. 



Open cells, washed in water after treatment 



Cells with perforated cappings compared with open 



cells. 



!a.s 



1^ 



1-1.9 



Open cells 



it 



II 



3! 



I 



SI'S 



+J MO tj 



1^ 



Sealed cells 





gfel 





'II 





mi 



s.a us 



Third. 



Do. 

 Fifth. 

 Fourth. 



Do. 

 Third. 



Fourth 



■ Two showed few spores germinated. 

 > One showed tew siwres germinated. 

 ' Seven ^owed few spores germinated. 



Table 10. — Cultural results of various tests with samples of comi treated for '. 

 hours in water-formalm solution 



•a 

 m <o 



o " o 



Hi 



1^ 



•sa 



Open cells 





II 



a 



II 



3 



•3 

 o 





.ogo 

 ■8 &3 



+5 boo H 



]s,auB 



Sealed cells 



n 



o 





sg^a-. 





Open cells only; used solution 



Open cells only; fresh solution 



Fresh solution; control for samples washed after 



treatment. 



Open cells washed in water after treatment. 



Cells with perforated cappings compared with open 



cells. 



First., 



Seventh. 



' Oermination of few spores; doubtful in the case of two cultures. 

 ■ Showed few spores germinated. 



CAFPINGS REMOVED WITH KNIFE 



Considerable experimenting with various methods of opening cap- 

 pings sufficiently to allow easy access of solutions into the cells dem- 

 onstrated that the only satisfactory way is to use an uncapping knife 

 and actually to cut away the cappings, as is done when uncapping 

 honey, before the combs are placed in the disinfectant solution. If 

 the knife is sharp and well heated, brood cells may be as easily un- 

 capped as honey cells, leaving a sharp, complete opening and adding 

 but little to the labor factor in handling the combs. If the knife is 



