34 CIECULAR 3 9 2, U. S. DEPARTMENT OF AGBICULTUEE 



sample in a wooden (fig. 21) or strong cardboard box. Do not me 

 tin, glass, or waxed paper. 



Smears of dead brood and small crushed pieces of comb are tre- 

 quently unsatisfactory for diagnosis but will be examined in case 

 the foregoing instructions cannot be followed. 



HOW TO SEND SAMPLES OF ADULT BEES 



(1) Select, if possible, bees that are sick or recently dead; bees 

 that have been dead for some time are not satisfactory for examina- 

 tion. (2) Send at least 50 bees in a sample; if poisoning by a.r- 

 senicals is suspected, 200 or more bees will be needed for analysis. 

 (3) Send bees in a wooden or strong cardboard box and not in tin 

 or glass. 





Figure 21. — How to send a sample of brood for laboratory examination. 

 HOW TO SEND SAMPLES OF TREATED COMB 



(1) Send a sample not less than 4 by 5 inches in size if in- 

 fection is heavy, or an entire brood comb if infection is slight. (2) 

 Brood remains should be present in abundance. (3) Pack the comb 

 in a clean wooden box as soon after treatment as possible. (4) 

 Do not send sannples that contain honey. 



HOW TO ADDRESS SAMPLES 



All samples should be addressed to the Bee Cultttee Laboratokt, 

 Bttreatj of Entomology and Plant Quarantine, National Agri- 



CTJLTDRAL EeSEARCH CeNTEE, BeLTSVILLE, Md. 



Your name and address should be plainly written on the box. 

 If the sample is forwarded by an inspector, his name and address 

 should also appear on the box. 



