24 



Recipes : Solution No 1 — for sprinkling, disinfecting, etc. — half a tea- 

 spoonfnl of soluble creolin in a 'itre of wat^r. 



Solution No, 2 For washing hives, pla'.fnrnas, etc. — two teaspoonfuls of 

 soluble creolin to a litre of water. 



Solution No. 3— for feeding — a quarter of a teaspoonful of soluble 

 creolin in a litre of syrup. 



The water of the syrup ought always to be poured upon the top of the 

 creolin and thoroughly mixed with it ; and the mixture should b'l well shaken 

 before using. 



Use. Prepare a hive and a proper floor board, which has been washed 

 with solution No. 2. Then, after having taken out the comb from th» infected 

 hive, shake off the befs, and sprinkle the comb with solution No. 1. Take 

 out all superfluous comb and spray it with solution No. 2, and extract the 

 honey from it. The honey can then be boiled, and if it is used for feeding 

 the bees, it can be diluted and phenol added in the proportion of one quarter 

 to a teaspoonful to a litre of the diluted honey. The combs are then put 

 back and the bees fed with medicated syrup. If the bees take the syrup, the 

 dose can be gradually increased ; but we must be careful not to give more 

 than one teaspoonful to a litre of syrnp. If the bees refuse to touch it, 

 which is not at all improbable, if they have access to other food, pour the 

 melicated syrup upon the neighboiing comba, when the bees will quickly 

 become habituated to it, and afterwards will take it in the ordinary manner. 

 The vapour of creolin also acts as a disinfectant. A small phial of concen- 

 trated creolin may be placed in a comer of the hive, and lightly stopped 

 with a cotton plug ; and the lower part of the cotton being in contact with 

 the liquid, capillarity will take place and draw up the creolin, and the heat 

 of the hive will produce the necessary evaporation. . A piece of blotting 

 paper can be used by saturating it with creolin, and placing it upon the floor 

 board or in a box covered with perforated zinc, so that the bees will not come 

 into contact with the disinfectant. 



Creolin is neither poisonous nor corrosive for man ; but, in strong doses, it 

 kills insests. Consequently it is necessary not to give greater strengths than 

 those mentioned above. In the use of this remedy it is necessary to stimu- 

 late the production of brood by feeding liberally with medicated syrnp ; if 

 the disease does not yield to this trpatment, the queen should be removed. 



Experiments on the antiseptic value of creolin. a. Sloped agar — each 

 tube, inoculated with one loopful of spores, was plugged with cotton wool, 

 saturated with creolin, and then cipped with lead foil Tubes were kept at 

 22" C. and 37" C. 



Result : After four days at 22° C. — No growth, except beneath the condensa- 

 tion water in the tubes. 

 After four days at 37° C. — No growth. 

 At the end of (his time new cotton plugs were inserted iiito the tubes in the 

 place of the creolin ones, and the cultures again incubated, when good growth 

 ensued in 24 hours, 



h. Agar platfs were made and streaked with two loopfuls of spores. In 

 each plate was placed a square inch of thick blotting paper, with four drops 

 of creolin on it. The plates were kept in the incubator at 37° C, and re- 

 moved in 48 hours, when very slight growth was manifest. On removal of 

 the creolin and further incubation of the plates, good growth was obtained. 

 Control plates gave copious growth. These experiments were repeated with 

 only one drop of creolin. 



Result, after 24 hours — abundant growth. With two drops of creolin, 



