INFECTIOUS DISEASES 297 



drawn into sterile bottles, the size of which will be determined by the 

 demand. The bottles are properly sealed, labeled, and wrapped, and 

 until needed put back in storage at a temperature not to exceed 50 

 degrees Fahrenheit. 



The foregoing description is the general method of production of 

 anti-hog-cholera serum. Many little factors that are of considerable 

 importance have not been enumerated. The accompanying illustra- 

 tions may convey some idea of the problems of the production of 

 this product. The production of anti-hog-cholera serum has become 

 of -such vast importance to the swine industry of the country that 

 Congress has provided means for its regulation in order that a potent 

 serum may be obtained. 



SERUM PRODUCTION 



The following is a general description of requirements for serum 

 production. 



LOCATION OP BUILDINGS 



Location to be such that surroundings will not interfere with sanitary 

 production and that their operation will not contaminate surrounding areas. 



CONSTRUCTION OW BUILDINGS 



The construction of buildings to be of material that will permit of daily 

 cleaning and disinfection, stone, brick, tile, and concrete being especially 

 recommended. 



ARRANGEMENT OF PLANT 



1. The arrangement and equipment of buildings to be such as to insure 

 the production of a pure and uncontaminated product. 



2. Swine to be kept in separate buildings located a sufficient distance 

 from the building containing the bleeding (either virus or serum) and 

 hyperimmunizing rooms in which swine are to be placed for the latter 

 purpose only. 



3. The serum bleeding room, the virus bleeding room, and the hyper- 

 immunizing room to be separate and distinct rooms. 



4. The preparation of serum, and the preparation of virus from the whole 

 blood, to be operations conducted in separate and distinct rooms without 

 direct communication with each other or with the foregoing not larger than 

 an opening which will permit the transfer of receptacles containing the 

 freshly drawn blood, and to be used for no other purpose. 



5. Separate washing and sterilizing rooms to be provided. 



6. The prepared serum and virus to be stored in properly cooled rooms. 



7. Swine kept for virus production and the testing of serum to be prop- 

 erly isolated so as to reduce to a minimum the danger of spreading infec- 

 tion. Separate and distinct feed rooms to be provided for such animals. 



8. Mortuary, crematory, abattoir, and desiccating or rendering plant 



