xxiv INTEODUCTION. 



injecting ■warm salt-solution. The following apparatus is 

 necessary, and must all be placed on the table ready for use 

 before the animal is killed ; for the blood coagulates after 

 death so rapidly as to allow no time to look for instruments. 



1. Cannulse ; glass ones are the most convenient; they 

 should be of various sizes, to fit the several vessels which it is 

 proposed to, inject, and each should have a slight constriction 

 near the tip, so that it may be firmly secured in the vessel by 

 a ligature. The largest cannula that will enter the vessel 

 should be used. 



2. A glass syringe, capable of holding about an ounce of 

 fluid. A larger syringe is liable to get blocked up. 



3. Several pieces of strong india-rubber tubing, about an 

 inch long, to connect the nozzle of the syringe with the 

 cannula, both of which they must fit rather tightly. 



4. Two or three glass plugs to fit the india-rubber tubing. 



5. A dish of water or salt-solution, in which all the cannulas 

 and india-rubber connections are laid, to displace the air from 

 them. 



6. Pine plaster of Paris. 



7. A mortar and pestle. 



8. Colouring matter, such as vermilion previously rubbed 

 up with water : it must be well shaken before use. 



9. A piece of muslin to strain the injection through. It 

 must be well wetted before use. 



10. A jar into which to strain the injection. 



11. A jar containing about half a pint of salt-solution (-75 

 per cent.), at a temperature of 38° to 40° C. 



12. Dissecting-board, pins, scalpels, scissors, two pairs of 

 forceps, seeker, thin string or thread, and two or three pairs 

 of ' bull-dogs.' These last are very short spring-forceps, the 

 spring being so arranged as to close the forceps. They are 

 convenient to stop the escape of blood or injection from any 

 vessels that may have been cut. 



The injection should be performed close to a large sink, 

 over which is a water-tap with a foot or two of wide india- 

 rubber tubing attached. 



When everything is ready, kill the animal, and as soon as 



