APPENDIX 



LIST OF EEAGENTS. 



I. REAGENTS FOR KILLING, HARDENING, AND 

 PRESERVING. 



ABSOLUTE ALCOHOL. 



Use, — Hardening reagent, but most used for dehydrating speci- 

 mens before clearing them in oil of cloves or turpentine. 



ALCOHOL (Spirit). 



It is used of the following strengths : 90 per cent., 70 per cent., 

 50 per cent., 30 per cent. 



Preparation. — 90 per cent, alcohol is the ordinary 'methy- 

 lated spirit.' The wealjer solutions are obtained by 

 diluting it with water. 



Use. — (1) For preserving specimens ; not weaker than 70 per 

 cent. 



(2) Hardening reagent ; 90 per cent. 



(3) For dissolving the intercellular substance of certain 

 tissues, so that the cells may be isolated by teasing ; 

 30 per cent. 



CHKOMIC ACID, 10 per cent, solution. 



Preparation. — Dissolve 10 grms. chromic acid crystals in 

 100 CO. water. 



