460 APPENDIX. 



Use. — As a fixing and hardening reagent it must be reduced to 

 a 1 per cent, or '5 per cent, solution by addition of water 

 before use ; and osmic acid may be added to it. 



CORROSIVE SUBLIMATE, solution. 



Prepa/raUon.Satwskte water with mercuric cliloride : 1 litre 



dissolves about 70 grms. 

 Use. — (1) As a hardening reagent it is used cold. 



(2) For killing small animals it may be used warm or 

 cold. 



After use it should be washed out in alcohol to which a 

 little tincture of iodine is added. 



CORROSIVE-ACETIC, mixture. 



Preparation. — Take 90 vols, of concentrated subUmate solution 



and add 10 vols, of glacial acetic acid. 

 Use. — An excellent fixative for general work. Wash out in 



alcohol. 



FIEMMING'S SOLUTION. 



Preparation. — Fifteen parts chromic acid (Ip. c), 4 parts osmic 



acid (2 p. c), 1 part glacial acetic acid. 

 Use. — Good fixative and hardening reagent. Wash well in 



water. 



MULLER'S FLUID. 



Preparation.— Dissolve 25 grms. potassium bichromate and 

 10 grms. sodium sulphate in 1 litre water. 



Use. — Hardening reagent. It also dissolves intercellular sub- 

 stance, so that the cells may be separated by teasing. 



OSMIC ACID (OsO^), 2 per cent, solution. 



Preparation. — Dissolve 1 grm. ' osmic acid ' crystals in 50 c.c. 



distilled water. Keep in the dark. 

 Use. — For kOling microscopic animals the vapour is a good 



fixatiA-e. Before using the solution, it is to be diluted with 



1 to 3 times its bulk of water. 



