462 APPENDIX. 



II. BEAGENTS USED IN STAINING. 



ALUM CARMINE. 



Prepa/ration. — Dissolve 200 grms. ammonia alum in water : 

 boil 10 to 20 minutes with excess of carmine : filter : make 

 up to 1 litre with water : add a few drops of carbolic acid 

 to preserve from mould. 



Use. — Stain for hardened tissues. It may be diluted with 4 

 times its bulk of water or less. 



BOSAX CARMINE (Grenacher's). 



Prepa/ration. — ^Dissolve 12'5 grms. carmine and 20 grms. borax 

 in 500 c.c. water : add 500 e.c. 70 per cent, alcohol : allow 

 to stand two or three days : filter. 



Use. — Stain for tissues which have been hardened. May be 

 used warm (50° C.) or cold. The tissues should afterwards 

 be treated with acid alcohol. For staining objects which 

 are to be mounted whole, it should be diluted with several 

 times its bulk of 40 per cent, alcohol, and the treatment 

 with acid alcohol should be prolonged. 



ACID AICOHOL. 



Prepa/ration. — To 100 c.c. of 70 per cent, alcohol add '25 c.c. 



hydrochloric or nitric acid. 

 Use.— For differentiating the parts of stained specimens, and 



for removal of surplus stain. 



FICROCARMINE. 



Prepaa-ation. — Dissolve 10 grms. carmine in 40 c.c. strong 

 ammonia and 2 litres water. Add 50 grms. picric acid. 

 Shake well a few minutes : allow to stand : and decant. 

 Allow to stand a few days, stirring occasionally. Evapo- 

 rate to dryness over a water bath. To every 2 grms. of dry 

 residue add 100 c.c. water. 



C7«e.— Stain. It may be used with advantage for specimens 

 hardened with osmic acid or otherwise. Subsequent im- 



