LIST OF EEAGENTS. 463 



mersion for a short time in acid alcohol improves the effect 

 in the case of some tissues. If the ohjeot be dehydrated 

 rapidly, or by means of alcohol containing picric acid, a 

 double-stained effect may be obtained, some tissues being 

 stained yellow and others red. 



MEYER'S ACID HJEMALTJM. 



Preparation. — Dissolve 1 grm. haematein in 50 c.c. 90 per cent. 



alcohol. Dissolve 50 grms. alum in 1 litre distilled water. 



Mix and allow to settle. Add 20 c.c. glacial acetic acid 



and a crystal of carbolic acid or thymol. 

 Use. — For staining tissues in bulk or sections on the slide. In 



either case the object must be brought down to water, and 



after staining washed in 1 per. cent, alum-solution. 

 It is preferable to Kleinenberg's solution in being (1) 



of definite composition, (2) less affected by light, and (3) 



keeps better. 



METHYLENE BLTTE. 



Preparation. — Dissolve ^^ grm. in 100 c.c. salt-solution. 



Use. — For staining small animals in hfe. It brings out the 

 gland-ceUs, muscle-cells, and nerve elements. A small 

 quantity should be diluted with twenty times its bulk of 

 distilled water. 



DAHLIA, -25 per cent, solution. 



Preparation. — Dissolve ^ grm. in 100 c.c. distilled water. 

 Use. — For staining Protozoa and cover-glass preparations. 



SAFBANIN. 



Preparation. — Dissolve ^-1 grm. in 100 c.c. water or 50 per 



cent, alcohol. 

 Use. — A nuclear stain for the same objects as Dahlia. 



