464 APPENDIX. 



III. CLEARING EEAGENTS 



(i.e. EBAGENTS FOR EEMOVING ALCOHOL OE PARAFFIN, AND 

 EENDEEING THE OBJECT PEEMBABLB BY BALSAM). 



OIL OF CLOVES. 



Use. — To remove alcohol from an object before mounting it in 

 balsam. The object must be left in the oil tiU it becomes 

 transparent : if left too long the object may become 

 brittle. It is liable to destroy the colour of objects stained 

 with hsematoxylin. 



OIL OF CEDAR-WOOD. 



Use. — Same as oil of cloves. It is less liable to injure speci- 

 mens stained with hematoxylin, and is also useful for 

 clearing before imbedding. 



TUBPENTINE. 



Use. — To remove paraffin from sections (see Introduction, 

 p. xxxii), which shpuld only remain in it long enough for 

 the paraffin to be dissolved. 



CREASOTE AND TURPENTINE. 



Preparation. — Mix 100 c.c. creasote with 400 c.c. turpentine : 



allow to stand tiU clear. 

 Use. — Same as turpentine, but must not be used for sections 



cemented down with shellac. 



IV. MOUNTING MEDIA. 



SALT SOLUTION, -75 per cent. 



Preparation. — Dissolve 7"5 grms. common salt in 1 litre 



water. 

 Use. — Living or fresh tissues are usually examined in it. 



GLYCERIN.— Used either pure, or diluted with an equal bulk of 

 water. 

 Use.— For njpj;nting microscopical objects, 



