466 APPENDIX. 



SOFT PABAFFIN. 



Melting-point about 40° C. 



TJse. — To smear the sides of the block that is being cut, so as 

 to ensure the sections cohering by their edges into a con- 

 tinuous ribbon. 



VI. CEMENTS EOE FIXING DOWN SECTIONS 

 CUT IN PAEAFP^IN. 



GLYCEEIN AND ALBUMEN. 



Preparation. — Mix equal volumes of fresh white of egg and 

 glycerin. Keep a piece of camphor in the bottle. 



Use. — For fixing sections in order upon the slide. For full 

 directions see Introduction, p. xxxi, where directions for 

 staining sections on the slide are also given 



SHELLAC AND ALCOHOL. 



Preparation. — Dissolve perfectly diy bleached shellac in ab- 

 solute alcohol : allow to stand two or three weeks : 

 decant. Keep in a bottle with a cap ground to fit. 

 Dilute with absolute alcohol when it becomes too thick to 

 flow evenly. 



Hue. — For fixing sections in order upon the slide. If the film 

 becomes white and opaque on drying, warm it to 60° C. 

 till the opacity disappears. 



COLLODION AND OIL OF CLOVES. 



Preparation. — Mix equal parts of collodion and oil of cloves. 



Use. — Same as above. The slide is smeared very sUghtly with 

 the mixture and the sections laid upon the fihn while still 

 moist. Then proceed as with shellac and oil of cloves. 

 If the solution becomes too stiff, dilute with ether. 



