432 APPENDIX 



HAUANN'S CARMINE. 



Prepa/ration. — Dissolve 30 grms. carmine in 240 c.o. strong 

 ammonia : add glacial acetic acid drop by drop till neutral : 

 allow to stand four weeks : decant and filter. 



The deposit may be redissolved and the solution neu- 

 trahsed as before, the product being preferable to the 

 solution first obtained. 



TJse. — Stain for hardened tissues. It may with advantage be 

 used warm. t is especially useful for animals like the 

 leech, which are liable to become too hard if treated with 

 borax carmine and acid alcohol. 



HaiMATOXYIIN, Kleinenberg's solution. 



Prepa/ration. — Make two solutions, (a) and (b), as foUows. 



(a) Saturate 70 per cent, alcohol with crystaUised calcium 



chloride, and add powdered alum to saturation. 



(b) Saturate 70 per cent, alcohol with alum. 



Mix 100 c.c. solution (a) with 800 c.c. solution 

 (b) : allow to stand a week : decant and add a few 

 drops of saturated solution of hasmatoxyhn in abso- 

 lute alcohol. 



The solution should be of a deep violet-blue 



colour. If it be reddish, add a solution of sodium 



carbonate (not bicarbonate) drop by drop till the 



violet-blue colour is obtained. 



Use. — Stain. The specimen must be previously placed in 



strong alcohol. It wiU not give satisfactory results with 



tissues which contain acid. 



MAGENTA. 



Prepa/ration. — Dissolve "6 grm. crystaUised magenta (roseine) 

 in 1 litre water. Add 6 c.c. absolute alcohol. 



Use. — Stain. Used for fresh preparations. Not fitted for 

 specimens of which it is desired to make permanent pre- 

 parations. 



GOLD CHLOEIDE, 1 per cent, solution. 



Preparation. — ^Dissolve 1 grm. gold chloride in 100 c.c. water. 

 Use. — Stain for nerve-tissues. It may be diluted with an 

 equal bulk of water. 



