LIST OF REAGENTS 435 



VI. CEMENTS FOE FIXING DOWN SECTIONS 

 CUT IN PAEAFFIN. 



SHELLAC AND ALCOHOL. 



Preparation. — Dissolve perfectly dry bleached shellac in ab- 

 solute alcohol : allow to stand two or three weeks : decant. 

 Keep in a bottle with a cap ground to fit. Dilute with 

 absolute alcohol when it becomes too thick to flow evenly. 



Use. — For fixing sections in order upon the slide. For method 

 of using, see I'^troduction, p. xxx. If the film becomes 

 white and opaque on drying, warm it to 60° C. tUl the 

 opacity disappears. 



COLLODION AND OIL OF CLOVES. 



PrejiaraUon. — Mix equal parts of collodion and oil of cloves. 



Use. — Same as above. The slide is smeared very slightly with 

 the mixtm-e and the sections laid upon the film while still 

 moist. Then proceed as with shellac and cloves. 

 If the solution becomes too stiff dilute with ether. 



GLYCERIN AND ALBXTMEN. 



Preparation. — Mix equal volumes of firesh white of egg and 

 glycerin. Keep a piece of camphor in the bottle. 



Use. — Same as above. After clearing, the slide must be placed 

 in absolute alcohol for a few minutes and then returned to 

 turpentine. 



If desired, sections may be stained after they are fixed 

 to the slides. To do this, clear as before, transfer succes- 

 sively to absolute alcohol, 90 per cent, and 70 per cent, 

 spirit ; then stain, and transfer to the alcohol in the reverse 

 order (70, 90 per cent., absolute) : clear and mount. 



VII. INJECTION FLUIDS. 



CABUINE INJECTION FLITID. 



Preparation. — Eub up carmine in water : add ammonia drop 

 by drop tUl the carmine is dissolved : filter : evaporate at 

 a gentle heat, stirring constantly to get rid of the ammonia. 

 Eub up the residue with camphor water. 

 Use.-^For injecting smaU animals and parts of animals. It 

 must be well shaken before use. 



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