174 CLINICAL BACTERIOLOGY AND HEMATOLOGY 



xylol. The permanence of the preparation will depend on the 

 thoroughness with which this step is carried out. 



8. Balsam and a cover-glass. 



IV. Staining sections to demonstrate the tubercle bacillus ; 

 applicable to the leprosy bacillus also. 



I, 2, and 3. Xylol, alcohol, and water, as before. 



4. Carbol fuchsin heated until the steam rises for five minutes 

 or longer, care being taken that the section does not dry up. Or 

 the slide may be immersed in the stain and kept in a warm place 

 for twenty-four hours. 



5. Dilute sulphuric acid until only a faint pink tinge appears 

 after washing. This will generally require an immersion of ten 

 minutes or more. 



6. Methylene blue for three or four minutes. Some of the 

 stain comes out in the alcohol, so that the section must be stained 

 more deeply than will be required ultimately. 



7. Rinse off the blue stain in water, and then remove the greater 

 part of the latter with blotting-paper ; this is to render the dehy- 

 dration more rapid. 



8. Absolute alcohol, two lots in rapid succession. 



9. Xylol. 



10. Balsam and a cover-glass. 



