APPENDIX 



The following tables may be useful if stains, etc., have to be 

 prepared when the metric weights and measures are not at hand. 

 The equivalents given are not absolutely accurate, but are 

 sufficiently near for most purposes : 



I Litre - 35-J fluid ounces. 



100 c.c. 3^ fluid ounces. 



I Gramme i5"43 grains. 



I Fluid ounce - 28-4 c.c. 



I Fluid drachm 3-5 c.c. 



I Grain 0-064 gramme. 



To convert grammes per litre into grains per ounce, multiply by 

 the factor ^^-. Thus, 10 grammes per litre = 10 x ^'5 = 4-4 grains 

 per ounce. 



To convert cubic centimetres per litre into minims per ounce, 

 multiply by the factor if. Thus, 25 c.c. per litre = 25 xi|= 12 

 minims per ounce. (A close approximation is given by multiplying 

 by h) 



To convert degrees Centigrade into degrees Fahrenheit, multiply 

 by the factor f and add 32. 



II 



Neisser's Method of Staining the Diphtheria Bacillus. 



The following method has been placed in the Appendix, as 

 there is some doubt as to its value. It is a method by which the 

 so-called polar bodies are stained with methylene blue, while the 

 rest of the organism is coloured a faint yellowish-brown with 

 Bismarck brown. 



Cultures should be made on blood-serum, and should not be less 



245 



