436 Veterinary Medicine. 



attachment of the mesenteries. In Hanover the diaphragm, in- 

 tercostals and masseters are chosen. In Magdeburg the diaphragm, 

 oculars, masseteric, cervical and costal. In the Bureau of Ani- 

 mal Industry the pillar of the diaphragm, psoas, inner side of the 

 shoulder and base of the tongue. The material taken should be 

 muscular tissue from close to its termination in tendon or fascia, 

 which obstructing the progress of the wandering trichina, favors 

 its eucystraent at this point. 



Identification of Specimens. The pieces of muscle (each 

 about the size of a hazel-nut) taken from one carcase must be 

 placed in small tin boxes, that are then marked on the lid with 

 the name of the muscle and a number which is stamped or tagged 

 on the carcase from which the specimen was taken. The catcase 

 may be split from nose to croup, but must not be further muti- 

 lated until it has been passed upon and tagged. The specimens 

 are locked in a box and sent to the microscopist, who must exam- 

 ine them one by one according to number and make a report of 

 the numbers borne by all trichinous specimens. 



Examination of Specimens. In trichinosis of old-standing, 

 with calcified capsules, these stand out as white specs on the red 

 muscles, and are easily detected by the naked eye. Even the un- 

 calcijSed capsules can often be seen with the aid of a lens. Place 

 a piece of muscle, i mm. in thickness, in a solution of caustic pot- 

 ash (i:8), or acetic acid, in a watch glass for a few seconds until 

 it has become clear, and look through it toward a black back- 

 ground. The capsules stand out as whitish specs. If the solu- 

 tion is left too long in contact, the capsules themselves become 

 transparent and the test is no longer possible. Any such speci- 

 men must be taken as merely indicative and the presence of tri- 

 chinae must be verified under the microscope. 



Microscopic Examination. This may be made under a 

 compressor easily with alow magnifying power (lo to 30 dia- 

 meters) or more carefully with a higher (100 diameters). In any 

 case the glass slides and covers must be carefully cleaned to avoid 

 mistaking vegetable or other fibers for trichinae. 



For the low power, thin stripes are cut from the muscle, parallel 

 to the direction of the fibers, and five or six such pieces are laid 

 side by side on a rather thin glass slide. This is covered by a 

 similar .slide and the two are firmly pressed together by screws at 



