Species of Anopheles 45 



Department. The determination of species found 

 was made by Mr. F. Knab. Detailed studies 

 of the relation to malaria of Isthmian Anopheles 

 were made by Dr. S. T. Darling, " and portions of 

 his published work are quoted. 



In order to have sufficient adults for the experi- 

 mental work, the sanitary inspectors along the line 

 of the Canal sent pupae and well developed larvae 

 to the laboratory daily. Anopheles larvae and 

 pupae are delicate organisms and must not be 

 subjected to rough treatment. They were trans- 

 ported in wide-mouthed jars, not too many in a 

 jar, containing about an inch of water and shaded 

 from the sun. If several inches of water are placed 

 in the temporary container and subjected to the 

 direct rays of the sun, or if the larvae are too much 

 overcrowded and subjected to constant shaking, 

 many will die. By having shallow water but slight 

 effort is needed for the larvae to reach the surface. 

 In collecting larvae in the field white enameled 

 saucers or dippers were found convenient, as the 

 larvae are easily visible against a white back- 

 ground. 



In connection with the experimental work con- 

 ducted at the laboratory, it became necessary to 

 collect live adult Anopheles from houses at the 



' See his Studies in Relation to Malaria. 



