METHODS OP USING CULTURE MEDIA 



m 



One method of using gelatin and also agar is as " puncture" 

 or "stab" cultures. The tubes (the narrower tubes are to be 



Fig. 115. — Round wire basket. 



Fig. 116. — Squarp wire basket. 



Fig. 117. — Culture tubes with media in them. X |. 1 to 7 are the 

 smaller tubes mentioned in the text; 9 the larger tube; 8 is extra large for 

 potato tubes; 1, plain broth; 2, plain milk; 3, litmus milk; J/., gelatin for 

 "stab" or "puncture" culture; 5, agar for "stab" or "puncture" culture; 

 6, agar for "slope" or "slant" culture; 7, blood serum; 8, potato tube; 

 9, agar for plating. Note the transparency of the broth and gelatin and 

 the slight opalescence of the agar. 



