METHODS OF USING CULTURE MEDIA 175 



Ized. Inoculation is made to the bottom and anaerobes 

 grow well (Fig. 124). 



D. For slope or plate, or any type of surface cultures the 

 Novy jar (Fig. 125) is the most practical device. It is good 



Fig. 124. — Deep tubes showing anaerobic growth. 1, shows a few small 

 gas bubbles; S, shows the medium broken up by the excessive development 

 of gas. 



practice to combine the vacuum method, the hydrogen 

 replacement method and the oxygen absorption method in 

 using these jars. In operation a solution of 20 per cent. 

 NaOH is poured on the bottom of the jar to a depth 

 of 1 or 2 cm., the cultures are placed on glass supports 

 above the alkali and a short wide tube of strong pyrogallol 

 is set in on the bottom in such a way that it may be easily 



