CH. IX] OF TRANSMUTATION 121 



growth from the culture IBj, was injected into the peritoneal 

 cavity of a guineapig. The animal was found dead the next 

 morning and a pure culture of B. typTwsus was obtained from 

 the heart's blood. From the peritoneal fluid and spleen was 

 obtained, in addition to B. typhosus, a coliform organism 

 possessing the following characters : a gram-negative motile 

 bacillus, forming acid and gas in glucose, mannite, lactose and 

 dulcite, but producing no change in salicin or cane sugar but 

 giving rise in the neutral red medium to gas and fluorescence, 

 not liquefying gelatin or forming indol in broth and giving an 

 acid reaction in litmus milk without any clotting. A broth 

 culture from the original agar slope was carefully tested but 

 typical B. typhosus alone found. The broth culture was 

 planted on agar and the experiment repeated with a loopful 

 of this growth. The animal did not die and a pure culture 

 of B. typhosus was recovered from the peritoneal cavity. 



(e ii) (Page 230, exp. 6.) One standard loopful of a 24 hours' 

 growth from the culture IB2 was then injected into the peri- 

 toneal cavity of a guineapig in the same manner. The animal 

 was found dead next morning and a pure culture of B. typhosus 

 was obtained from the heart's blood and spleen. From the 

 peritoneal fluid was obtained, in addition to B. typhosus, a 

 coliform bacillus. 



(eiii) (Page 231, exp. 7.) The urine of the typhoid carrier 

 " I " was again plated after having been kept over 14 months 

 in a flask. A colony was again planted on agar and a standard 

 loopful of the growth again injected into the peritoneal fluid 

 of a guineapig. The animal was found to be dying the next 

 morning and was killed with chloroform and a pure culture 

 of B. typhosus was obtained from the heart's blood. From the 

 peritoneal fluid and spleen a pure culture of a coliform or- 

 ganism was obtained. The latter organism failed to produce 

 any typhoid agglutinins when injected into a rabbit, or to ab- 

 sorb agglutinins from a known typhoid serum. 



The last experiment was repeated, the same strain being 

 used (" I ") after 14 days further growth on agar. The injection 

 did not prove fatal to the guineapig and from the peritoneal 

 fluid a pure culture of B. typhosus was obtained. 



