154 BACTERIOLOGY. 
150 (Behring). Cholera spirilla grown in bouillon con- 
taining no peptone or only 0.5 per cent. of peptone are 
destroyed in half an hour by 0.1 per cent. of hydro- 
chloric acid; grown in 2 per cent. peptone-bouillon 
their vitality is destroyed in the same time on the 
addition of 0.4 per cent. HCl. In any case the organ- 
isms to be tested should all be treated in exactly the 
same way and the results accompanied by a statement 
of the conditions under which the tests were made. 
The following table gives the results and methods 
used in an actual experiment to test the effect of blood- 
serum upon the disinfecting action of bichloride of 
mercury and carbolic acid upon bacteria: 
Test FOR THE DIFFERENCE OF EFFEct OF BICHLORIDE OF 
MERcURY AND CarBoLic Acip SoLurIONS ON ANTHRAX AND 
TypHoIp BaciniI in SeruM AND BovuiLuon. 
; ‘ I, 1 |1%| 2 
Time. 1/8] 5 {10/20 30/45] 2 |,174| 2 
A. Serum. .. .2.5¢.¢. 
Solution equals 
HgCl, sol. 1:1000 2.5 c.c. |+)+/+/+/+/-|4+| —} —] — Lin 2000 
: dichloride. 
Anthrax thread 
B. Bouillon. . . 2.5¢.c¢. 
HgCl, sol. 1: 1000 2.5¢.c. |—|—|——|-|—|—| — | — | — | Same. 
Anthrax thread 
C.Serum., .. .25cc¢. 
Solution equals 
Carbolic sol. 5 p.ct. 2.5 ce. |+)+/+/+/+)+/—| —| + |—| 2% per cent. 
carbolic acid. 
Typhoid threads 
D. Bouillon. . . 2.5¢.¢ 
Carbolic sol. 5 p.ct. 2.5 c.c. |-+/+/+,+/+/+/—-] — | — | — | Same. 
Typhoid threads 
— Indicates total destruction of bacteria with no growth in media. 
+ Indicates lack of destruction of bacteria with growth in media. 
