490 BACTERIOLOGY. 
ments the serum and culture were injected subcutane- 
ously in rabbits at the same time, but in opposite sides 
of the body : 
TABLE SHOWING STRENGTH OF AVERAGE GRADE OF ANTI- 
STREPTOCOCCIC SERUM GIVEN BY SELECTED HORSES AFTER 
SIX MONTHS OF INJECTION OF SUITABLE AMOUNTS OF 
Livine STREPTOCOCCI. 
Weight 
of _ Amounts Result. | Autopsy. 
rabbit. inoculated. 
Serum and culture: Grms. | Serum. Cult. 
1. Inoculated at same time; 1480 | 0.25 ¢.c. 0.01 cc. | Lived 
2s e m 1350 | 0.125 ‘** 0.01 ‘* ‘s 
3. te 4 a 1600 | 0.25 “ 0.01 “ * 
4. Subcutaneously ‘ 1440. | 0.25 “ 0.01 * en 
5. On opposite sides ‘“ 1770 | 0.1 ‘ OO1 * Ke 
6. ee “et te its 1630 0.1 “i 0.01 “e “ 
Controls : 
1. Rabbits injected ees 1750 0.001 ‘* | Died in | Strept, in- 
culture only ‘ 4 days, fection. 
# 1870 0.001 “| Died in “8 ss 
24 hrs. 
3. oe oY 1820 0.01 “ | Died in “ fe 
4 days 
The above results have been repeatedly obtained, and 
are absolutely conclusive that, as Marmorek and others 
have claimed, the serum of properly selected animals, 
which have been repeatedly injected with living strep- 
tococci in suitable doses, possesses bactericidal properties 
upon the same streptococcus when it comes in contact 
with it within the bodies of animals. 
Definite protection from the serum has been obtained 
by many reliable observers since Marmorek’ s first 
reports. 
