INTRACELLULARIS MENINGITIDIS. 517 
never appears within the nucleus and rarely within 
other cells (Fig. 67). 
Tt stains with all the ordinary aniline colors, but best 
with Loffler’s methylene-blue. According to Weich- 
selbaum, it is decolorized by Gram’s solution; Jaeger 
states that this is not constantly the case. 
Fie. 67. 
Diplococcus intracellularis meningitidis. >< 1100 diameters. 
Biological Characters. It does not grow at room-tem- 
perature, but only in the incubator, and its develop- 
ment is usually scanty on the surface of agar, but some- 
times a few colonies grow quite vigorously. It does 
not grow at all or scarcely any in bouillon, and very 
scantily in bouillon plus one-third blood-serum. It 
grows comparatively well on Léffler’s blood-serum 
medium as used for diphtheria cultures. 
- When grown on nutrient agar or glycerin-agar, at 
the end of forty-eight hours in the incubator, there 
develops a tolerably good growth, appearing as a flat 
layer of colonies, about one-eighth of an inch in diam- 
