564 BACTERIOLOGY. 
thrax bacilli in culture and the bacilli of malignant 
cedema in the bodies of animals are frequently seen to 
do. Under the hanging drop the bacilli are observed 
to be actively motile, and in stained preparations flagella 
may be demonstrated surrounding the periphery. The 
spores are elliptical in shape, usually thicker than the 
bacilli, lying near the middle of the rods, but rather 
toward one extremity. This gives to the bacilli con- 
taining spores a somewhat spindle shape. 
Stains with the ordinary aniline dyes, but not with 
Gram’s method, or only with difficulty and after long 
treatment or intense colors. 
Biological Characters. Like the bacillus of malignant 
cedema this is also a strict anaérobe, and cannot be cul- 
tivated in an atmosphere in which oxygen is present. 
It grows best under hydrogen, and does not grow under 
carbonic acid. This bacillus develops at the room-tem- 
perature in the usual culture media, in the absence of 
oxygen, but it grows best in those to which 1.5 to 2 per 
cent. of glucose or 5 per cent. of glycerin has been added. 
Growth on Agar. The colonies on agar are some- 
what more compact than those of malignant oedema, but 
they also send out projections very often. In agar stick 
cultures, in the incubator, growth occurs after a day or 
two also some distance below the surface, and is accom- 
panied by the production of gas and a peculiar disagree- 
able acid odor. 
Pathogenesis. The bacillus of symptomatic anthrax 
is pathogenic for cattle (which are immune against ma- 
lignant cedema), sheep, goats, guinea-pigs, and mice; 
horses, asses, and white rats when inoculated with a cul- 
ture of this bacillus present only a limited reaction; and 
rabbits, swine, dogs, cats, chickens, ducks, and pigeons 
